Journal: iScience

Article Title: Mitochondria-targeted cancer analysis using survival and expression: Prioritizing mitochondrial targets that alleviate pancreatic cancer cell phenotypes

doi: 10.1016/j.isci.2024.110880

Figure Lengend Snippet: Mitochondrial fusion is dispensable for cell proliferation (A) Western blotting of PANC-1 cells treated with non-targeting control or two different MFN1 siRNAs for 5 days. An arrow and an asterisk indicate MFN1 and non-specific bands, respectively. (B) Quantification of band intensity (mean ± SD, n = 3). (C) PANC-1 cells were transfected with the indicated siRNAs and subjected to laser confocal immunofluorescence microscopy with anti-PDH antibodies. The boxed regions are magnified. (D) Cell proliferation of the transfectants was examined by a crystal violet assay (mean ± SD, n = 5). (E) PANC-1 cells were infected with lentiviruses expressing scramble or DRP1 shRNAs and then treated with DMSO or MYLS22 for 24 h. Knockdown of DRP1 was confirmed by Western blotting. (F) Quantification of band intensity (mean ± SD, n = 3). (G) Mitochondria were analyzed by immunofluorescence microscopy with anti-TOM20 antibodies. (H) Cell proliferation was analyzed by a crystal violet assay (mean ± SD, n = 5). ANOVA with post-hoc Tukey in (B, D, F, H): ∗ p < 0.05, ∗∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: siRNA: MFN1 #1 , Thermo Fisher Scientific , Assay ID: s31218.

Techniques: Western Blot, Control, Transfection, Immunofluorescence, Microscopy, Crystal Violet Assay, Infection, Expressing, Knockdown

Journal: iScience

Article Title: Mitochondria-targeted cancer analysis using survival and expression: Prioritizing mitochondrial targets that alleviate pancreatic cancer cell phenotypes

doi: 10.1016/j.isci.2024.110880

Figure Lengend Snippet:

Article Snippet: siRNA: MFN1 #1 , Thermo Fisher Scientific , Assay ID: s31218.

Techniques: Recombinant, Modification, Ligation, Transfection, Infection, Electron Microscopy, Protease Inhibitor, Negative Control, shRNA, Sequencing, Plasmid Preparation, Software

Journal: iScience

Article Title: Mitochondria-targeted cancer analysis using survival and expression: Prioritizing mitochondrial targets that alleviate pancreatic cancer cell phenotypes

doi: 10.1016/j.isci.2024.110880

Figure Lengend Snippet:

Article Snippet: The following Silencer Select siRNAs from Thermo Fisher were used: negative control (4390843), MFN1 #1 (s31218), MFN1 #2 (s31220), MIC60 #1 (s21633), and MIC60 #2 (s21634).

Techniques: Recombinant, Modification, Ligation, Transfection, Infection, Electron Microscopy, Protease Inhibitor, Negative Control, shRNA, Sequencing, Plasmid Preparation, Software

Journal: iScience

Article Title: Mitochondria-targeted cancer analysis using survival and expression: Prioritizing mitochondrial targets that alleviate pancreatic cancer cell phenotypes

doi: 10.1016/j.isci.2024.110880

Figure Lengend Snippet: Mitochondrial fusion is dispensable for cell proliferation (A) Western blotting of PANC-1 cells treated with non-targeting control or two different MFN1 siRNAs for 5 days. An arrow and an asterisk indicate MFN1 and non-specific bands, respectively. (B) Quantification of band intensity (mean ± SD, n = 3). (C) PANC-1 cells were transfected with the indicated siRNAs and subjected to laser confocal immunofluorescence microscopy with anti-PDH antibodies. The boxed regions are magnified. (D) Cell proliferation of the transfectants was examined by a crystal violet assay (mean ± SD, n = 5). (E) PANC-1 cells were infected with lentiviruses expressing scramble or DRP1 shRNAs and then treated with DMSO or MYLS22 for 24 h. Knockdown of DRP1 was confirmed by Western blotting. (F) Quantification of band intensity (mean ± SD, n = 3). (G) Mitochondria were analyzed by immunofluorescence microscopy with anti-TOM20 antibodies. (H) Cell proliferation was analyzed by a crystal violet assay (mean ± SD, n = 5). ANOVA with post-hoc Tukey in (B, D, F, H): ∗ p < 0.05, ∗∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: siRNA: MFN1 #2 , Thermo Fisher Scientific , Assay ID: s31220.

Techniques: Western Blot, Control, Transfection, Immunofluorescence, Microscopy, Crystal Violet Assay, Infection, Expressing, Knockdown

Journal: iScience

Article Title: Mitochondria-targeted cancer analysis using survival and expression: Prioritizing mitochondrial targets that alleviate pancreatic cancer cell phenotypes

doi: 10.1016/j.isci.2024.110880

Figure Lengend Snippet:

Article Snippet: siRNA: MFN1 #2 , Thermo Fisher Scientific , Assay ID: s31220.

Techniques: Recombinant, Modification, Ligation, Transfection, Infection, Electron Microscopy, Protease Inhibitor, Negative Control, shRNA, Sequencing, Plasmid Preparation, Software